I'm on my second playthrough (NG+1) and I'm trying to farm gold cards, however I'm missing both black ant worker and soldier...traveled to the ant hill to farm them and ALL of the black ants are gone...?

The only ones left on my map are the ones on the shed porch... anyone else having this issue?

I haven't seen this reported at all. By any chance did you happen to harm the black ant queen in your first playthrough? If you did, all black ant spawns are drastically reduced due to your actions. If you didn't I recommend submitting a support ticket, and we'll look into it. 

Obsidian Support

Good afternoon, the same thing has happened to me, I am in NG+1 and the ants in the black anthill do not respawn, there are two soldier ants that are there and if you kill them they respawn, but no more, neither the ones in the surroundings nor the ones around the black anthill and the ones in the red anthill continue to respawn normally even though I have killed their queen.
I moved to NG+2 thinking that the problem would be fixed but no, now the black queen is infected in both anthills but in the red one normal red ants continue to emerge as well as infected ones but in the black anthill there are only the two soldier ants that always respawn and all the others are infected.

I've contacted support and sent them the last save file of the game so they can investigate it, let's see if there's any luck.